Analysis Note

ControlFull-length ISL1-hIgGFc transfected HEK293 cell lysate

Application

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in paraffin-embedded human lung cancer and cervical carcinoma tissues.

Optimal working dilutions must be determined by end user.

Research CategoryStem Cell Research

Research Sub CategoryPluripotent & Early Differentiation

Detect ISL1 using this mouse monoclonal antibody, Anti-ISL1 Antibody, clone 1H9 validated for use in western blotting, Immunofluorescence & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.

Immunogen

Purified recombinant fragment of human ISL1 expressed in E. Coli. corresponding to human ISL1.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in full-length ISL1-hIgGFc transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~93 kDa observed. Uncharacterized bands may appear in some lysate(s).